ABSTRACT
RESUMO Objetivo avaliar a prevalência de depressão, ansiedade, estresse e qualidade do sono de profissionais da enfermagem na pandemia da COVID-19. Método estudo quali-quantitativo realizado com 104 profissionais de um hospital privado do Paraná, Brasil, em julho de 2020. Utilizaram-se três instrumentos para a coleta de dados: Escala de Depressão, Ansiedade e Estresse (DASS-21), Questionário de Avaliação do Sono Leeds (LSEQ) e Índice da Qualidade do Sono de Pittsburgh (PSQI). Resultados foram encontrados 48% sinais de depressão, 52% sinais de ansiedade e 52% sinais de estresse nos profissionais. Quanto à qualidade de sono, 75% da população apresentou distúrbios do sono, 68% relatam insônia com uma média do PSQI de 6,88. Conclusão apesar do percentual de profissionais com distúrbio do sono e estresse apresentarem-se inferiores comparados à literatura, os níveis de ansiedade, insônia e depressão foram maiores, corroborando com o impacto da pandemia na saúde mental de profissionais da enfermagem.
RESUMEN Objetivo evaluar la prevalencia de depresión, ansiedad, estrés y calidad del sueño de profesionales de enfermería en la pandemia de COVID-19. Método estudio cuali-cuantitativo realizado junto a 104 profesionales de un hospital privado en Paraná, Brasil, en julio de 2020. Para la recolección de datos, se utilizaron tres instrumentos: Escala de Depresión, Ansiedad y Estrés (DASS-21), Cuestionario de Evaluación del Sueño de Leeds (LSEQ) y el Índice de Calidad del Sueño de Pittsburgh (PSQI). Resultados se encontraron 48% de signos de depresión, 52% de signos de ansiedad y 52% de signos de estrés en los profesionales. En cuanto a la calidad del sueño, el 75% de la población presentó trastornos del sueño, el 68% refirió insomnio con un PSQI medio de 6,88. Conclusión a pesar del menor porcentaje de profesionales con trastornos del sueño y estrés en comparación con la literatura, los niveles de ansiedad, insomnio y depresión fueron más altos, corroborando el impacto de la pandemia en la salud mental de los profesionales de enfermería.
ABSTRACT Objective to assess the prevalence of depression, anxiety, stress and the sleep quality among nursing professionals during the COVID-19 pandemic. Method this quali-quantitative study was conducted with 104 professionals from a private hospital in Paraná, Brazil, in July 2020. Data were collected using three instruments: the Depression, Anxiety and Stress Scale (DASS-21), the Leeds Sleep Evaluation Questionnaire (LSEQ), and the Pittsburgh Sleep Quality Index (PSQI). Results 48% of the personnel showed signs of depression, 52% signs of anxiety and 52% signs of stress. As for sleep quality, 75% had sleep disorders, and 68% reported insomnia with a mean PSQI of 6.88. Conclusion although the percentage of personnel with sleep and stress disorders was smaller than in the literature, the levels of anxiety, insomnia and depression were higher, corroborating the impact of the pandemic on the mental health of nursing personnel.
ABSTRACT
Objetivo: avaliar a prevalência de depressão, ansiedade, estresse e qualidade do sono de profissionais da enfermagem na pandemia da COVID-19. Método: estudo quali-quantitativo realizado com 104 profissionais de um hospital privado do Paraná, Brasil, em julho de 2020. Utilizaram-se três instrumentos para a coleta de dados: Escala de Depressão, Ansiedade e Estresse (DASS-21), Questionário de Avaliação do Sono Leeds (LSEQ) e Índice da Qualidade do Sono de Pittsburgh (PSQI). Resultados: foram encontrados 48% sinais de depressão, 52% sinais de ansiedade e 52% sinais de estresse nos profissionais. Quanto à qualidade de sono, 75% da população apresentou distúrbios do sono, 68% relatam insônia com uma média do PSQI de 6,88. Conclusão: apesar do percentual de profissionais com distúrbio do sono e estresse apresentarem-se inferiores comparados à literatura, os níveis de ansiedade, insônia e depressão foram maiores, corroborando com o impacto da pandemia na saúde mental de profissionais da enfermagem.
Objective: to assess the prevalence of depression, anxiety, stress and the sleep quality among nursing professionals during the COVID-19 pandemic. Method: this quali-quantitative study was conducted with 104 professionals from a private hospital in Paraná, Brazil, in July 2020. Data were collected using three instruments: the Depression, Anxiety and Stress Scale (DASS-21), the Leeds Sleep Evaluation Questionnaire (LSEQ), and the Pittsburgh Sleep Quality Index (PSQI). Results: 48% of the personnel showed signs of depression, 52% signs of anxiety and 52% signs of stress. As for sleep quality, 75% had sleep disorders, and 68% reported insomnia with a mean PSQI of 6.88. Conclusion: although the percentage of personnel with sleep and stress disorders was smaller than in the literature, the levels of anxiety, insomnia and depression were higher, corroborating the impact of the pandemic on the mental health of nursing personnel.
Objetivo: evaluar la prevalencia de depresión, ansiedad, estrés y calidad del sueño de profesionales de enfermería en la pandemia de COVID-19. Método: estudio cuali-cuantitativo realizado junto a 104 profesionales de un hospital privado en Paraná, Brasil, en julio de 2020. Para la recolección de datos, se utilizaron tres instrumentos: Escala de Depresión, Ansiedad y Estrés (DASS-21), Cuestionario de Evaluación del Sueño de Leeds (LSEQ) y el Índice de Calidad del Sueño de Pittsburgh (PSQI). Resultados: se encontraron 48% de signos de depresión, 52% de signos de ansiedad y 52% de signos de estrés en los profesionales. En cuanto a la calidad del sueño, el 75% de la población presentó trastornos del sueño, el 68% refirió insomnio con un PSQI medio de 6,88. Conclusión: a pesar del menor porcentaje de profesionales con trastornos del sueño y estrés en comparación con la literatura, los niveles de ansiedad, insomnio y depresión fueron más altos, corroborando el impacto de la pandemia en la salud mental de los profesionales de enfermería.
ABSTRACT
Abstract Eucalyptus species possess anti-inflammatory, antifungal, antibacterial, and insecticidal properties. In this study, the chemical composition and biological activities of Eucalyptus cinerea essential oil (EO) and the leaf and stem anatomy were investigated. EO was extracted by Clevenger apparatus and the compounds were identified by GC/MS. The antioxidant activity was evaluated by DPPH, ABTS, and reducing phosphomolybdenum complex. Broth microdilution was used to determine antimicrobial activity. Cytotoxicity was verified against HeLa, HRT-18, and Calu-3 cells by MTT assay. The cytotoxic mechanism was studied by cell DNA content, cell cycle, and DNA fragmentation. The microscopic analyzes of the leaves and the stems were performed by light microscopy, field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. The main constituent of the EO was 1,8-cineole (55.24%). The EO showed low antioxidant and antimicrobial activities. Calu-3 cells showed a significant reduction in viability with IC50 of 689.79 ± 29.34 μg/mL. EO at 1000 μg/mL decreased the DNA content in Jurkat cells. In general, EO increased cell percentage in sub-G0 and S phases with concomitant reduction of cell percentage in G0/G1 and G2/M phases and provided DNA fragmentation of 29.73%. Anatomical and micromorphological features of the leaves and stems can help in the species identification and its differentiation from other Eucalyptus species.
Subject(s)
Terpenes , Biological Phenomena , Oils, Volatile , Myrtaceae , MicroscopyABSTRACT
Abstract Curcumin (CUR) shows potential use for treating cancer. However, CUR has low solubility and reduced bioavailability, which limit its clinical effect. Therefore, the development of nanocarriers can overcome these problems and can ensure the desired pharmacological effect. In addition, it is mandatory to prove the quality, the efficacy, and the safety for a novel nanomedicine to be approved. In that sense, this paper aimed (a) to prepare CUR-loaded polyethylene glycol-poly(ε-caprolactone) nanocapsules; (b) to validate an analytical method by high performance liquid chromatography (HPLC) for quantifying CUR in these nanoformulations; (c) to evaluate the physicochemical stability of these formulations; and to investigate their cytotoxicity on NIH-3T3 mouse fibroblast cells. The HPLC method was specific to CUR in the loaded nanocapsules, linear (r = 0.9994) in a range of 10.0 to 90.0 µg.mL-1 with limits of detection and quantification of 0.160 and 0.480 µg.mL-1, respectively. Precision was demonstrated by a relative standard deviation lower than 5%. Suitable accuracy (102.37 ± 0.92%) was obtained. Values of pH, particle size, polydispersity index, and zeta potential presented no statistical difference (p > 0.05) for CUR-loaded nanoparticles. No cytotoxicity was observed against NIH-3T3 mouse embryo fibroblast cell line using both the tetrazolium salt and sulforhodamine B assays. In conclusion, a simple and inexpensive HPLC method was validated for the CUR quantification in the suspensions of nanocapsules. The obtained polymeric nanocapsules containing CUR showed suitable results for all the performed assays and can be further investigated as a feasible novel approach for cancer treatment.
Subject(s)
Animals , Mice , Curcumin/pharmacology , Embryonic Stem Cells/drug effects , Fibroblasts/drug effects , Chromatography, High Pressure Liquid , Toxicity Tests , Nanotechnology , NIH 3T3 Cells , Embryo, Mammalian/cytology , NanocapsulesABSTRACT
Abstract Metallic nanoparticles have great potential as a chemotherapeutic agent. The aim of this study was to develop and characterize silver and gold nanoparticles using a simple method, as well as evaluating the potential cytotoxic activity in relation to the K-562 cell line. For the synthesis, a solution containing the metallic ions was subjected to magnetic stirring with the aqueous extract of Lavandula dentata L. and a change of colour was observed. With the data obtained from the analyses we concluded that the nanoparticles were successfully obtained by a simple and green method using the aqueous extract of L. dentata. The obtained nanoparticles presented a reduced size, a low level of polydispersion, and a homogenous spherical shape. The nanoparticles presented intense and characteristic diffraction peaks, which could be correlated to the planes of the centred cubic structure of the silver and gold. The two formulations presented predominantly crystalline characteristics. The infrared analysis suggested that the amides and alcohols present in the samples may have been responsible for the reduction and limitation of the size and dispersion of the silver and gold nanoparticles. The cytotoxic assay showed that the nanoparticles demonstrated great potential to reduce the cell viability of the K-562 cell line, especially the gold nanoparticles.
Subject(s)
Leukemia, Myeloid , Lamiaceae/toxicity , Cytotoxins , Metal Nanoparticles/analysisABSTRACT
Abstract The objective of this study was to evaluate the effect of liraglutide, an analog of glucagon-like peptide-1 (GLP-1) in association with physical exercise, on the metabolic and biochemical parameters of rats induced to obesity with a cafeteria diet. Male Wistar rats, aged 21 days, were randomly divided into: Controls (CON) receiving standard feed and water ad libitum; and obese (OBESE) receiving cafeteria diet ad libitum, added to the standard diet. Groups were then subdivided into: Liraglutide animals that received subcutaneous injections of liraglutide from 80 to 90 days of life; exercised (EXE) animals submitted to swimming sessions, three days a week (15 min); and liraglutide + EXE animals that received liraglutide in association with physical exercise. Treatment with liraglutide reduced deposits of mesenteric and periepididymal fat, HOMA-IR, triglycerides, glucose and insulin in obese group. It is important to note that the association of the two treatments reduced the body weight in animals, deposits of mesenteric and periepididymal fat, HOMA-IR, blood triglyceride levels, glucose and insulin in obese rats. As such, the association of liraglutide with exercise potentiated the effects of the drug and ameliorated obesity pathology more effectively. retirar
Subject(s)
Animals , Metabolic Syndrome , Liraglutide/therapeutic use , Motor Activity , Obesity/drug therapy , Rats, WistarABSTRACT
Tendo em vista o aumento exorbitante de sobrepeso e obesidade na infância, este estudo tem como propósito verificar as possíveis diferenças existentes em relação ao sobrepeso e obesidade em crianças de 7 a 10 anos de idade estudantes da rede particular de ensino do município de Ponta Grossa-Pr, através da utilização de três métodos classificatórios. Metodologia: Este estudo foi realizado com 189 crianças com idades entre 7-10 anos. A amostra foi classificada em sobrepeso e obesidade de acordo com a distribuição percentilar proposta nos critérios de MUST et al., COLE et al. e CDC. A análise foi realizada de forma descritiva, obtendo-se a classificação nominal das prevalências de sobrepeso e obesidade. Resultados: Com base nos resultados encontrados para o sexo masculino, observou-se que há diferença entre os três métodos em todas as idades, principalmente na classificação de sobrepeso, com exceção da faixa etária de 8 anos. Para o sexo feminino pôde-se notar que as diferenças entre os três métodos não foram tão marcantes, com exceção da faixa etária de 7 anos de idade. Porém, ao totalizar os índices de sobrepeso e obesidade nesta faixa etária, notou-se semelhança, ficando clara a falta de concordância em relação à definição de sobrepeso e obesidade. Conclusões: Pode-se concluir que há falta de concordância entre os métodos utilizados para classificar sobrepeso e obesidade em crianças.
Considering the exorbitant increase of overweight and obesity in the childhood, this study has as purpose to verify the possible existent differences in relation to the overweight and obesity in children with ages among 7 and 10 years, students from particular schools of the city of Ponta Grossa-Pr, through the use of three qualifying methods. Methods: This study was accomplished with 189 children and the sample was classified in overweight and obesity in agreement with the distribution percentilar proposed in the criteria of MUST et al., COLE et al. and CDC. The analysis was accomplished in a descriptive way, being obtained the nominal classification of the overweight prevalences and obesity. Results: With base in the results found for the masculine sex, it was observed that there is difference among the three methods in all ages, mainly in the overweight classification, except for the 8 year-old age group. For the feminine sex it could be noticed that the differences among the three methods were not so outstanding, except for the 7 year-old age group. However, when totaling the overweight indexes and obesity in this age group, it was noticed similarity, being clear the agreement lack in relation to the overweight definition and obesity. Conclusions: It can be concluded that there is agreement lack among the methods used to classify overweight and obesity in children.